RAW Tutorial

• Introduction
  • Overview
  • Requirements
  • Other useful materials
  • Notes
• Basic processing
  • Loading configuration files and images, creating subtracted scattering profiles, saving profiles
  • Guinier analysis
  • Molecular weight analysis
  • Saving analysis information
  • Kratky analysis
  • Similarity Testing
  • Basic SEC-SAXS processing
  • WAXS processing and merging
  • A few additional tricks
• Advanced processing
  • Pair-distance distribution analysis – GNOM in RAW
  • Pair-distance distribution analysis – BIFT in RAW
  • Assessing ambiguity of 3D shape information - AMBIMETER in RAW
  • 3D reconstruction with bead models – DAMMIF/N and DAMAVER in RAW
  • 3D reconstruction with electron density – DENSS in RAW
  • Aligning reconstructions with high resolution shapes
  • Advanced SEC-SAXS processing – Singular value decomposition (SVD)
  • Advanced SEC-SAXS processing – Evolving factor analysis (EFA)
  • Advanced SEC-SAXS processing – Baseline correction
• Creating a configuration file
  • Centering and calibration
  • Masking
  • Setting normalization and other options
  • Setting absolute scale with water
  • Setting absolute scale with glassy carbon
  • Setting a molecular weight standard